The identification of genes related to salinity stress tolerance in Pseudomonas sp. SWRIQ۱۱ (CECT ۳۰۷۴۱)

BACKGROUND AND ABJECTIVEIdentification of new halotolerant bacteria taxa with special beneficial features is increasingly needed. In this research the genome of SWRIQ۱۱ is assessed.MATERIALS AND METHODSThe whole genome sequencing was carried out by the Illumina HiSeq platform. The gene detection and genome annotation were done exploiting the Rapid Annotation using Subsystem Technology (RAST) server, NCBI Prokaryotic Genome Annotation Pipeline (PGAP), KEGG Automatic Annotation Server (KAAS), and UniProt database. Gene clusters related to secondary metabolites biosynthesis were determined using antiSMASH.RESULTS AND DISCUSSIONThe strain SWRIQ۱۱ can tolerate salinity up to ۶% w/v NaCl. The SWRIQ۱۱ inoculation did not induce necrosis in leaves in the hypersensitive response (HR) assay, and as a result, the strain is not considered phytopathogenic. Moreover, the strain had no hemolytic activity. The genome of the strain SWRIQ۱۱ contained an assembly size of ۶,۱۹۶,۳۹۰ bp with GC content of ۶۰.۱%. The genome of SWRIQ۱۱ was rich in genes related to stress response sigma factors, stress sensors, signaling and regulation proteins. Moreover, genes related to main adaptation mechanisms for tolerating salinity stress including synthesis of chaperons, exopolysaccharides, and osmoprotectants, ion homeostasis, nutrient acquisition, and antioxidants defenses were identified in the genome of SWRIQ۱۱. The results of genome analysis are in accordance with laboratory experiments and Pseudomonas sp. SWRIQ۱۱ is a halotolerant rhizobacteria. The genome of Pseudomonas sp. SWRIQ۱۱ contained the genes related to plant growth promotion, biocontrol agents, antitumor and herbicide capabilities, and biodegradation.CONCLUSIONIn conclusion, Pseudomonas sp. SWRIQ۱۱ is a potential safe halotolerant strain with different efficiencies like biocontrol, antitumor, herbicide, and biodegradation capabilities which must be further assessed.