The Prevalence of P۵۳ Mutations in Laryngeal Cancer in Kerman

Background &Aims: Laryngeal cancer is the second common cancer of respiratory tract, following the lung cancer. Carcinogenesis is a complex multistage process; molecular genetics has provided the evidence that activation of proto-oncogene and loss or inactivation of tumor suppressor genes (TSG) are involved in a large number of malignancies. One of the earliest significant tumor suppressor genes identified in head and neck squamous cell carcinoma (HNSCC) was P۵۳ have a role in growth suppression activities. Thus, when P۵۳ is deleted or silenced, the cell develops a selective growth advantage and becomes a cancer. Mutations in P۵۳ are correlated with poorer survival and response to treatment. The aim of this study was to survey the prevalence of P۵۳ gene mutation in patients with laryngeal cancer and to select an appropriate method of treatment. Methods: The samples were ۵۲ patients with laryngeal cancer diagnosis have been treated by surgery. Investigation of TP۵۳ mutation where performed by multiple ligation probe amplification (MLPA) technique which analyze the full length of gene from exon ۱ to ۱۲. Results: The TP۵۳ mutation was discovered in ۸۰.۸ percent of samples. By contrast between two main forms of mutation (i.e. deletions and duplications), we found that the deletions mostly occurred within exons ۱, ۳, ۶, ۹ and ۱۲ by ۵۹.۶ percent and duplications observed in exons ۱, ۲, ۷, ۸ and ۱۱ by ۲۱.۲ percent. Conclusion: Considering our results and reminding this fact that nowadays the definitive diagnosis of laryngeal cancer is made using biopsy and pathology techniques, we suggest that all biopsy specimens should be tested and those confirmed positive for TP۵۳ mutations need some further decisions by physicians.