Study of the antibiotic resistance pattern and frequency of beta lactamase and tetracycline resistance genes in Escherichia coli isolated from urinary tract infections in women in Tabriz city

Urinary tract infection (UTI) is one of the most prevalent diseases in human. Unfortunately, the indiscriminate use of antibiotics leads to resistance in bacteria gradually. The aim of this study was to determine the antibiotic resistance pattern and frequency of beta lactamase and tetracycline resistance genes in Escherichia coli isolated from urinary tract infections in women in Tabriz city. In this cross-sectional descriptive study, ۴۰ E.coli samples isolated from women with UTI were tested for determining the antibiotic resistance pattern as recommended by the Clinical Laboratory Standards Institute (CLSI) using disk diffusion method. Then, PCR method was performed for identification of tetA, tetB, tetC, TEM and SHV genes. The most antibiotic resistance was related to ampicillin and cephalexin (۱۰۰%) and the least antibiotic resistance was related to tetracycline (۲۵%). The frequency of tetA, tetB, tetC, TEM and SHV genes was ۵%, ۰%, ۰%, ۳۰% and ۰%, respectively. One E.coli isolate (۲.۵%) harbored TEM and tetA genes simultaneously. Regarding the high frequency of resistance to beta lactam and cephalosporin antibiotics, precise antibiogram testing is highly recommended before any antibiotic prescription in cases of UTI.