Investigation the expression Candida albicans EFG۱ gene in Vaginal Candidiasis and biofilm formation

Candida albicans has the ability to change between yeast and hyphal cells and is known to be a virulence property. Efg۱gene of C.albicans is as a main transcription factor that plays pivotal roles in biofilm formation The aim of the current study is to investigate the presence of Efg۱ gene in Candida albicans isolates from women with vaginal candidiasis and its impact on biofilm formation.We used ۵۰clinical isolates subjected to PCR-RFLP to confirm C.albicans. Total RNA was extracted from C. albicans isolates by glass bead and lysis buffer, and cDNA was synthesized using Reverse Transcriptase enzyme. RT-PCR was used to evaluate the expression of Efg۱ gene. Biofilm formation was evaluated in ۹۶-well microplate and then tetrazolium reduction was assayed. All data were analyzed using t-test .۵۰ clinical isolates out of ۱۵۰ were confirmed as C.albicans by using PCR-RFLP. ۴۷(۹۴%) isolates out of ۵۰ present Efg۱ gene by using PCR, and ۱۲(۲۵.۵۳%) isolates out of ۴۷ expressed Efg۱ gene by the RT-PCR. Isolates which have Efg۱gene successfully form dense biofilm on microplate. The results of the tetrazolium reduction assay test showed that ۲ isolates with Efg۱ gene expression and ۲ isolates lacked Efg۱ gene expression which was significant at P-value of۰.۰۱۴ (P<۰.۰۵). Results of MTT assay identified biofilm formation in isolates with Efg۱gene expression and no biofilm formation in isolates which no Efg۱ gene expression.It could then be concluded that the growth of hyphae is influenced by environmental factors which is also essential for biofilm formation.