Prevalence of recR gene in Campylobacter spp. isolated from domestic animals and water
سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 189
فایل این مقاله در 8 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJMCM-7-1_008
تاریخ نمایه سازی: 19 اسفند 1402
چکیده مقاله:
Although, many genes are involved in pathogenesis of Campylobacter, racR gene considered the main gene for Campylobacter pathogenicity in humans. The purpose of this study was determined the prevalence of recR gene in Campylobacter spp. isolated from domestic animals and water. To perform the study ۳۹۲ fecal and water samples were collected from poultry(۱۸۲), cow(۱۴۱), sheep and goat(۴۱) and water(۲۸). All samples were subjected for isolation of Campylobacter spp. using prêt KB method and the presumptive isolates authenticated by DNA sequencing of ۱۶srRNA genes. Finally, Campylobacter isolates assessed for detection of racR gene. The results obtained indicated that ۵۰ strains of Campylobacter spp. were isolated. High isolate frequency (۳۷/۵۰) was for Poultry and low frequency (۲/۵۰) was for sheep and goat. Of All isolates thirty six strains were identified as Campylobacter jejuni and the rest (۱۴ isolates) was Campylobacter coli. The genome of ۳۰(۸۳.۳%) C.jejuni and ۱۴(۱۰۰%) C.coli were contained racR gene. Hence based on foregoing evidence racR gene existed approximately in all isolates. Therefore, Campylobacter strains isolated from different sources could be considered infectious agent for human. It is because phenotypical character inducted by racR gene (colonization and heat tolerance) helps them to survive and cause infection.
کلیدواژه ها:
نویسندگان
hamid mahmoodipour
Department of Microbiology, Fars Science and Research Branch, Islamic Azad University, Fars, Iran Department of Microbiology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
majid Baserisalehi
Department of Microbiology, Kazeroun Branch, Islamic Azad University, Kazeroun, Iran
Amir Emami
Department of Burn and Wound Healing Research Center, Shiraz University of medical sciences, Shiraz, Iran