Effects of explant type and Agrobacterium rhizogenes strains on hairy root induction and alizarin production in madder (Rubia tinctorum Ardakan)
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 25
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شناسه ملی سند علمی:
JR_IJGPB-12-2_001
تاریخ نمایه سازی: 20 فروردین 1403
چکیده مقاله:
Hairy root culture of plants is a considerable way for the in vitro production of valuable metabolites because of genetic stability, rapid growth rate, biochemical stability, and high capacity in the synthesis of secondary metabolites. Alizarin is an anthraquinone derived from the roots of madder (Rubia tinctorum) and has been used since ancient times as a natural red dye and exhibits various pharmacological and biological activities including anticancer, antioxidant, and anti-microbial activity. The influence of two factors including explant (leaf, internode, cotyledon) and strains of A. rhizogenes (۱۵۸۳۴, ۲۶۵۶, MSU, R۱۰۰۰) was tested on hairy root production of madder. All explants produced hairy roots with acceptable frequencies but leaf explants produced the highest number of roots per explant followed by cotyledons. The highest root induction rate (۱۰۰%) and the highest number of hairy roots per explant were obtained from leaf explants inoculated with ۱۵۸۳۴ and R ۱۰۰۰. Analysis of the PCR products showed the presence of a ۴۰۳ bp amplicon related to the specific reproduction of rolA gene in transgenic roots. Anthraquinone production was documented in transgenic roots but there was a significant difference between roots from different bacterial strains. As a brief result, the use of suitable bacterial strains and explants were effective factors for hairy root induction in madder.
کلیدواژه ها:
نویسندگان
ابراهیم دورانی
Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
ام البنین هنرمند
Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
مصطفی ولی زاده
Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
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