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مقالات فارسیISIکنفرانسهاژورنالها

Two Novel Amino Acid Insertions in The Helicobacter pylori rdxA Gene Detected in Gastric Paraffin Biopsies

محل انتشار: فصلنامه میکروب شناسی پزشکی ایران، دوره: 18، شماره: 1
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 17

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https://civilica.com/doc/1955896

شناسه ملی سند علمی:

JR_IJMM-18-1_007

تاریخ نمایه سازی: 27 فروردین 1403

چکیده مقاله:

Background and Aim: The biggest problem in the management of patients with H. pylori infections is resistance to the first-line antibiotic drug metronidazole. Several mechanisms underlie the resistance of H. pylori to metronidazole, and one of them is mutations in the rdxA gene encoding the nicotinamide adenine dinucleotide phosphate (NADPH) protein. Therefore, we characterized the rdxA gene amplified by PCR from gastric paraffin biopsies. Materials and Methods. This is an explorative study using ۳۴ gastric paraffin biopsies that were H. pylori positive in a real-time PCR assay. To analyze the rdxA gene, we conducted nested PCR of a gene fragment, followed by DNA sequencing to analyze amino acid changes and protein structure analysis. Results: Of the ۳۴ H. pylori positive paraffin biopsies, ۵ were positive for the rdxA gene fragment. The amino acid change analysis showed that the NADPH protein had substitutions, insertions, frameshifts, and premature stop codons. Two novel amino acid insertions were found. The protein structure analysis showed that the NADPH protein with substitutions had amino acid changes forming ۲ pockets for active sites. The NAPDH protein with substitutions and the ۲ novel insertions showed the loss of one pocket. Conclusion: The present study reports on the genetic analysis of the rdxA gene showing DNA sequence diversity. Two amino acid insertions in the NADPH gene are a unique finding, and their effect on antibacterial resistant phenotype needs to be investigated in future studies.

کلیدواژه ها:

Helicobacter pylori ، rdxA ، amino acid ، insertion

نویسندگان

Wilda Fadila

Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia

Mardiastuti H. Wahid

Department of Microbiology, Faculty of Medicine, Universitas Indonesia-Cipto Mangunkusumo Hospital, Jakarta, Indonesia

Fadilah Fadilah

Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia

Rike Syahniar

Department of Microbiology and Parasitology, Faculty of Medicine and Health, Universitas Muhammadiyah, Jakarta, Indonesia

Ening Krisnuhoni

Department of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia-Cipto Mangunkusumo Hospital, Jakarta, Indonesia

Andi Yasmon

Department of Microbiology, Faculty of Medicine, Universitas Indonesia-Cipto Mangunkusumo Hospital, Jakarta, Indonesia

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