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مقالات فارسیISIکنفرانسهاژورنالها

In vitro evaluation of Yazd honey effect on Leishmania major [MRHO/IR/۷۵/ER] promastigotes

محل انتشار: فصلنامه میکروب شناسی پزشکی ایران، دوره: 18، شماره: 1
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 19

نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:
https://civilica.com/doc/1955900

شناسه ملی سند علمی:

JR_IJMM-18-1_003

تاریخ نمایه سازی: 27 فروردین 1403

چکیده مقاله:

Background and Objective:Leishmaniasis is a parasitic illness carried by the bite of some species of sand flies. The cause of the disease is protozoan parasites of the genus leishmania. This study investigated In vitro evaluation of Yazd honey effect on Leishmania major [MRHO/IR/۷۵/ER] promastigotes in stationary and logarithmic phases. Methods: Multifloral honey obtained by Yazd 's experienced beekeepers (Yazd, Iran) in the ۲۰۱۶–۲۰۱۷ harvest season was included in the study. The L. major strain, catalogued as [MRHO/IR/۷۵/ER], was cultured in BALB/c mice. We extracted amastigotes from the spleens of these mice, which we then converted into promastigotes within Novy-MacNeal-Nicolle (NNN) medium. The L. major strain was then carefully supplied at various times, and the parasite populations within the specified culture were monitored and quantified using both slide microscopy and enzyme-linked immunosorbent assay (ELISA) techniques. We started with a consistent amount of parasites and placed them into vials with screw-caps filled with ۵ml of a liquid medium. This medium was infused with honey at various levels, including ۶.۲۵, ۱۲.۵, ۲۵, and ۵۰ micrograms per milliliter. These vials were then incubated for several durations: starting from zero and continuing at ۶, ۱۲, ۲۴, ۴۸, ۷۲, and ۹۶ hours. Results:  The results of the counts taken were measured against those of the control group and showed that parasite load reduced with increasing Yazd honey concentrations (۶.۲۵ to ۵۰µg/ml) and contact duration (۶-۹۶ hours). Conclusion: The findings revealed that honey has the capacity to curb the growth and survival of parasites, positioning it as a potential subject for research in both animals and humans.

کلیدواژه ها:

Leishmania major ، Honey ، Yazd ، Viability ، in vitro

نویسندگان

Ali Fattahi Bafghi

Department of Parasitology and Mycology, Infectious Diseases Research Center, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Gilda Eslami

Department of Parasitology and Mycology, Infectious Diseases Research Center, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Hamideh Emtiazi

Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Mohammad Reza Mozayan

General Courses Department, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Elmira zarei

Department of Hematology and Blood Banking, School of Allied, Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran

Parisa Bagheri

Department of Hematology and Blood Banking, Paramedical School, Gerash University of Medical Sciences, Gerash, Iran

Elham Rezaee

Department of Medical Parasitology and Mycology, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Medical Parasitology and Mycology, Paramedical School, Gerash University of Medical Sciences, Gerash, Iran

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