Investigation of Isotherm, Thermodynamic, and Kinetics of Bovine Serum Albumin Adsorption onto MCM-۴۱@LDH
سال انتشار: 1403
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 27
فایل این مقاله در 13 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_ANALCH-11-2_005
تاریخ نمایه سازی: 30 اردیبهشت 1403
چکیده مقاله:
The survey of protein adsorption is important due to significant application of proteins in food, medicine, biotechnology, environment, and separation science. Therefore, in the current investigation, the adsorption of bovine serum albumin (BSA) onto a functionalized mesoporous (MCM-۴۱) using layered double hydroxide (MCM-۴۱@LDH) was investigated under different conditions such as the amount of adsorbent, pH of the solution, contact time, and protein concentration. The optimization process was carried out using central composite design (CCD). After characterization of modified mesoporous, more investigations were conducted to assess the adsorption of BSA onto MCM-۴۱@LDH, including adsorption isotherm, kinetics, and thermodynamic. It was revealed that Freundlich isotherm and second-order kinetics provided the best fit for support of MCM-۴۱@LDH. The values of Gibbs free energy (ΔG°), enthalpy (ΔH°), and entropy (ΔS°) were determined as -۳۱۰۰.۴۷ kJ mol-۱ K-۱ (at ۲۹۸.۱۵ K), -۳۱۰۱.۱۲ kJ mol-۱, and -۰.۰۰۲ kJ mol-۱, respectively. According to CCD analysis and desirability function of ۱.۰, the optimal conditions for BSA adsorption were determined to be a pH value of ۴.۵۰, BSA concentration of ۲۵۰ mg L-۱, support amount of ۰.۰۱۶ g, and contact time of ۵۵ min. The obtained findings can be used for purification, separation, and removal of BSA from complex samples.
کلیدواژه ها:
نویسندگان
Forough Karami
Department of Chemistry, Yasouj University, Yasouj ۷۵۹۱۸-۷۴۸۳۱, Iran. Central Research Laboratory, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Ardeshir Shokrollahi
Department of Chemistry, Yasouj University, Yasouj ۷۵۹۱۸-۷۴۸۳۱, Iran
Salimeh Abbasi
Department of Chemistry, Yasouj University, Yasouj ۷۵۹۱۸-۷۴۸۳۱, Iran