Comparison of immunochromatographic rapid test withmolecular method in diagnosis of canine parvovirus
محل انتشار: فصلنامه طب دامی ایران، دوره: 7، شماره: 1
سال انتشار: 1392
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 674
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شناسه ملی سند علمی:
JR_IJVM-7-1_008
تاریخ نمایه سازی: 26 اردیبهشت 1394
چکیده مقاله:
BACKGROUND: Canine parvovirus (CPV) infection is one of the most common causes of infectious gastroenteritis in dogs and is a highly contagious, often fatal disease. The original virus (CPV type 2) has had some mutations since its emergence and new variants (CPV-2a, 2b and 2c) have been reported from many countries all around the world. Early diagnosis and treatment can profoundly affect the disease outcome. OBJECTIVES:To comparethe ability of Immunochromatographic (IC) test to detect CPV infection in 50 PCR positive samples (n=50) with regard to virus strains. METHODS: 50 rectal swabs (n=50) were prepared from suspicious dogs and subjected to PCR and IC test respectively. RESULTS: The sensitivity of IC test in PCR positive samples was 84% (42 out of 50 samples) and the positive predictive value of the test was 100%. Using PCR, CPV strains in our study were 2a (18/50, 36%) and 2b (32/50, 64%) with the predominance of 2b strain. IC test was also able to diagnose 15/18 (83.3%) of CPV- 2a and 27/32 (84.3%) CPV-2b strain positive samples, which means IC test can detect CPV infections caused by both virus strains (2a and 2b), without significant difference. CONCLUSIONS:This study shows that IC test results are relatively reliable for diagnosing CPVinfection in daily veterinary practice and the test is able to diagnose both CPV-2a and CPV-2b which are prevalent strains in Iran
کلیدواژه ها:
نویسندگان
Sh Mohyedini
Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Sh Jamshidi
Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
S Rafati
Molecullar Immunology and Vaccine Research Laboratory, Pasteur Institute of Iran, Tehran, Iran
Gh.R Nikbakht
Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran