Effects of acrylamide on sperm parameters, chromatin quality, and the level of blood testosterone in mice
محل انتشار: مجله طب تولید مثل ایران، دوره: 12، شماره: 5
سال انتشار: 1393
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 541
فایل این مقاله در 8 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
این مقاله در بخشهای موضوعی زیر دسته بندی شده است:
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJRM-12-5_006
تاریخ نمایه سازی: 16 شهریور 1395
چکیده مقاله:
Background: Acrylamide (AA) is an important industrial chemical primarily. AA is also found in carbohydrate-rich foods that are prepared at high temperatures, such asFrench fries and potato chips. It is demonstrated that AA is a carcinogen and reproductive toxin and has ability to induce sperm damage.Objective: The aim of this study was to observe the effects of AA on sperm parameters and evaluation of sperm chromatin quality and testosterone hormone inmice. Materials and Methods: Totally, 16 adult male mice were divided into two groups. Mice of group A fed on basal diet; group B received basal diet and AA (10 mg/kg,water solution) for 35 days. The right cauda epididymis was incised and then placed in Ham’s F10 culture media at 37oC for 15 min. Released spermatozoa were used to analyze count, motility, morphology and viability. To determine the sperm DNAintegrity and chromatin condensation, the cytochemical techniques including Aniline blue, Acridine orange and Chromomycin A3 staining were used.Results: AA-treated mice had poor parameters in comparison with control animals. In sperm chromatin assessments, except TB (p=0.16), significant differences werefound in all of the tests between two groups. It was also seen a significant decrease in concentration of blood testosterone in AA-treated animals when compared tocontrols (p<0.001). Conclusion: According to our results, AA can affect sperm parameters as well assperm chromatin condensation and DNA integrity in mice. These abnormalities may be related to the reduction in blood testosterone
کلیدواژه ها:
نویسندگان
Majid Pourentezari
Department of Biology and Anatomy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Alireza Talebi
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Abulghasem Abbasi
Department of Biology and Anatomy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Mohammad Ali Khalili
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran