Melatonin improves development of early mouse embryos impaired by actinomycin-D and TNF-α
محل انتشار: مجله طب تولید مثل ایران، دوره: 12، شماره: 12
سال انتشار: 1393
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 385
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شناسه ملی سند علمی:
JR_IJRM-12-12_002
تاریخ نمایه سازی: 16 شهریور 1395
چکیده مقاله:
Background: Melatonin, a reactive oxygen species (ROS) scavenger and an antioxidant, has been shown that can inhibit apoptosis. Administration of melatonin may improve embryo development in assisted reproductive technology (ART). Objective: The aim of this study was to evaluate the role of melatonin in inhibitionof spontaneous and induced apoptosis by Tumor Necrosis Factor Alph (TNF-α) and actinomycin-D during preimplantation development of mouse embryos. Materials and Methods: Female BALB/c mice were superovulated with pregnant mare serum gonadotropin (PMSG) followed by human chorionic gonadotropin(HCG), then allowed to mate with male mice. The resultant 2-cell embryos weredivided into six groups as follows: control (group I), melatonin (group II), actinomycin-D (group III), actinomycin-D + melatonin (group IV), TNF-α (groupV), and TNF-α + melatonin (group VI). We recorded the numbers and developmental rates of the 4-cell, 8-cell, morula and blastocyst embryos. Blastocystswere stained with acridine orange in order to assess for the embryo quality. Results: The group IV showed a significantly higher developmental rate ofblastocysts compared to group III (p<0.05). The number of dead blastomers was significantly decreased in group IV in comparison to group III (p<0.05). Both V andVI groups had a lower developmental rate and lesser quality of blastocysts compared with group I. There was no significant difference in the developmental rate ofblastocysts from group II compared to group I (p<0.05). Conclusion: Supplementation of embryo culture media with melatonin can improve the quality and developmental rate of embryos. Melatonin can prevent cell death that was induced by TNF- α and actinomycine-D.
کلیدواژه ها:
نویسندگان
Behrooz Niknafs
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Ahmad Mehdipour
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Amaneh Mohammadi Roushandeh
Department of Anatomical Sciences, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.