Cytotoxic effects of zinc oxide nanoparticles biosynthesized with ceratonia siliqua l extract on liver cancer cells (hepg2) and normal cells (huvec)

Nanotechnology is a new branch of science with vast applications and nanoparticles with different levels of composition, size, and surface chemical properties can have different biological and medicalapplications. Nanoparticles typically have a higher percentage of atoms on their surface that cause them which increases their surface reactions. Proper design of nano materials can be used to target specific cancer cells. Previous studies have shown that different nanoparticles are capable of inhibiting cancer cells in several ways. So attention is focused on the synthesis of high-power nanoparticles for inhibiting cancer cells with the lowest toxicity and side effects on normal cells. In this study, the effect of Zinc Oxide Nanoparticles Biosynthesized with Ceratonia siliqua L (ZnO-NPs/Cs) toxicity on two normal and cancer cell lines was evaluated and compared. In this study, two cell lines including, Huvec cells as a normal and HepG2 cells as a cancerous cell lines were used. First, each cells was cultured in a specific culture medium with 1% FBS. After reaching the logarithmic phase, the cells were transferred to a culture plate and after 24 h were treated with a serial dilution doses of ZnO-NPs/Cs for 24h. For measure the inhibitory concentration (IC50) 15 μl of MTT were added to the cells, and after incubation (4h), the absorption was measured after adding DMSO solution. Analysis of the results showed that the ZnO-NPs/Cs at a concentration about 8μg/ml does not effect on normal cells , but shows the cytotoxic effect on Hep G2 cells and suppressed about 50% of the cells in this concentration. The IC50 during the incubation times of 24 h for normal and cancer cells were determined 7.8 and 31.2 μg/mL, respectively (P<0.05). Therefore, it can be said that cancer cells are inhibited at low concentrations of the nanoparticle, but these concentrations do not have a toxic effect on normal cells. Zin oxide nanoparticles biosynthesized with Amaranthus leaf water extract had the cytotoxic effect on cancer cells, but did not have toxic effects in normal cells. It seems that with further research in the future, it can be used to treat cancer