Investigation of Liraglutide Effects on Oogenesis in NMRI Mice

Background: In recent decades pregnancy is known as a fundamental problem for young couples. Type 2 diabetes is one of the infertility reason that for control and treatment of this disease many medications have been proposed. But many of them have serious complications on fertility. One of these drug is Liraglutide that is used to treat type 2 diabetes where have been shown that has various effects on different parts of body. Since the women may be suffering from type 2 diabetes, effects of this drug on the ovary is very important and necessary. In this research effect of Liraglutide on oogenesis in NMRI Mice in In vivo condition have been investigated.Methods: This study has been done on 30 adult female NMRI mice (8 to 12 weeks) that have been selected with body weight between 25 and 30 grams. Female mice were randomly divided into four groups: control, sham and two experimental groups. Experimental and sham groups were injected Liraglutide with 0.6 and 1.8 mg/ml density and solution for 30 days respectively and all were injected subcutaneously. The collected data were analyzed using one-way analyses and Tukey test.Result: The results of using Liraglutide has been shown marked decrease on number of Primordial follicles in experimental groups. Also the number of Primary, secondary, Graaf, atretic follicles, the corpus luteum and blood vessels have marked increase. In addition the diameter of primary follicles, corpus luteum and the blood vessels reduced. On the other hand the diameter of primordial, secondary and atretic follicles has shown marked increase.Conclusion: Regarding that using Liraglutide increases the number of secondary follicles and these follicles decrease time fertility limitation, therefore this drug cause to premature aging of the ovaries and reduce the probability fertility.