Investigation of Candida Rugosa Lipase Immobilization on the Modified Graphene Oxide

The attention to green methodologies have enlarged the usage of enzymatic technology in industrialprocesses. Enzyme immobilization.can lead to increase stability and improve the reusability of enzyme. In this project,candida rugosa lipase (CRL) was immobilized on the surface of modified graphene oxide nanosheets. Initially, thegraphene oxide (GO) was synthesized by Hummer method. Afterward, it was activated for covalent attachment ofenzyme by designing appropriate reaction based on the use of cyanuric chloride (CC) as an appropriate chemical linker.Several techniques such as XRD, FESEM and FTIR was applied to characterize nanosheets in various stages ofsynthesis and after immobilization of CRL enzyme. The optimized conditions were determined for the immobilizationprocess of CRL. The optimal time and initial concentration of CRL for immobilization at room temperature wererespectively specified about three hours and 47.42μg/mL. Under these conditions, the amount of immobilized CRL was14.46 μg per mg of support (GO@CC) and relative activity% was 97.99%.