Separation of Bovine serum albumin by green biocompatible cholinium aminoate-based aqueous two-phase systems containig polyethylene glycol di-methyl ether

Aqueous two-phase systems, ATPSs, are known as interesting media for the separation, extraction, and purification of some biomolecules ecpecially proteins. From this viewpoint, the ambition of this work is investigating ability of cholinium aminoate ([Ch][AA]) as novel, low toxic and high biodegradable ionic liquids including of cholinium glycinate, cholinium prolinate and cholinium valinate for inducing ABSs with polypropylen glycol, PPG, and then evaluating their performances for partitioning of bovine serum albumin, BSA, as a model protein. For achieving this purpose, binodal curves and tie-lines were experimentally measured at 298.15 K for four ABSs. Two empirical equations including Merchuk equation were applied in order to represent the binodal data. Tie-line were correlated with setschenow equation with a good performance. In addition, the partition behavior of BSA was studied by measuring of partition coefficient and extraction efficiency in mentioned systems.