Determination of Important Phenolic Compounds in Pakistani Brown Rice Varieties in Controlled, Germinated and Fermented Conditions by High Performance Liquid Chromatography
سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 303
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شناسه ملی سند علمی:
JR_PCBR-2-3_006
تاریخ نمایه سازی: 17 مهر 1398
چکیده مقاله:
A simple and novel high performance liquid chromatography (HPLC) method has been developed for the simultaneous determination and quantification of phenolic compounds in brown whole grain rice varieties of Pakistani origin. The accumulation of these compounds was studied during germination stages and fermentation process, thereby, providing a reliable and rapid method for their quantification in food samples. Calibration curves for the standard phenolic compounds showed good linear regression values (r2 =0.996-0.998) within the test ranges. The limit of detection and the limit of quantification were found in the range of 0.04-0.06 µg/mL and 0.166-0.205µg/mL, respectively. Precision (%RSD) of the method was found in the range of 0.05-5.25 and 0.05-0.58 for inter-day (n=3) and intra-day (n=5), respectively. The robustness (%RSD) was found in the range of 1.05-2.65. Excellent recoveries were attained within the range of 93%-106%. The average amount of phenolic compounds was found to be 0.185 g/100g in controlled condition whereas, further accumulation of these compounds was noticed during germination and fermentation phases. The maximum average amount of phenolic compounds after germination period of 120 hours and fermentation process was found to be 0.284 g/100g and 0.565 g/100g, respectively.
کلیدواژه ها:
نویسندگان
Amir Hayat
Institute of Advance Research Studies in Chemical Sciences, University of Sindh, Jamshoro-۷۶۰۶۲, Pakistan.
Taj Muhammad Jahangir
Institute of Advance Research Studies in Chemical Sciences, University of Sindh, Jamshoro-۷۶۰۶۲, Pakistan.
Muhammad Yar Khuhawar
Institute of Advance Research Studies in Chemical Sciences, University of Sindh, Jamshoro-۷۶۰۶۲, Pakistan.
Malik Alamgir
Institute of Advance Research Studies in Chemical Sciences, University of Sindh, Jamshoro-۷۶۰۶۲, Pakistan.
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