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مقالات فارسیISIکنفرانسهاژورنالها

Wear particles enhance autophagy through up-regulation of CD۱۴۷ to promote osteoclastogenesis

محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 21، شماره: 8
سال انتشار: 1397
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 136

فایل این مقاله در 7 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:
https://civilica.com/doc/1295244

شناسه ملی سند علمی:

JR_IJBMS-21-8_006

تاریخ نمایه سازی: 27 مهر 1400

چکیده مقاله:

Objective(s): The study aimed to uncover the underlying mechanism linking wear particles to osteoclast differentiation, and we explored the effect of titanium particles of different sizes on CD۱۴۷ expression and autophagy in macrophages. Materials and Methods: Effects of titanium particles on CD۱۴۷ and RANKL mRNA were detected by QPCR; protein level of CD۱۴۷ and Beclin-۱ were detected by Western blot; soluble RANKL were detected by ELISA. To determine the effect of CD۱۴۷ and autophagy, KG-۱a cells were transfected with siRNA-CD۱۴۷ or treated with autophagy inhibitor CQ (chloroquine), and then co-cultured with different sizes of titanium particles.Results: Our results showed that ۰.۲-۱.۲ µm and ۱.۲-۱۰ µm titanium particles up-regulate CD۱۴۷ to activate autophagy, which increase the level of soluble RANKL to promote osteoclastogenesis. Suppression of CD۱۴۷ with siRNA could diminish particle-induced autophagy and soluble RANKL expression. In addition, CQ could dramatically reduce particle-induced soluble RANKL expression. Conclusion: Our findings suggested a possible mechanism underlying wear debris-induced osteolysis and identified CD۱۴۷ as a potential therapeutic target in aseptic loosening.

کلیدواژه ها:

Autophagy ، CD۱۴۷ ، Osteoclastogenesis ، Peri-implant osteolysis ، RANKL

نویسندگان

Baohua Su

Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China, ۵۱۰۱۲۰

Deng Li

Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China, ۵۱۰۱۲۰

Jie Xu

Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China, ۵۱۰۱۲۰

Yingbin Zhang

Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China, ۵۱۰۱۲۰

Zhiqing Cai

Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China, ۵۱۰۱۲۰

Max Kauther

Department of Orthopedics and Trauma Surgery, University Hospital Essen, University of Duisburg-Essen, Hufelandstrasse ۵۵, ۴۵۱۴۷ Essen, Germany

Ruofan Ma

Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China, ۵۱۰۱۲۰

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