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مقالات فارسیISIکنفرانسهاژورنالها

Effects of insulin-like growth factor-induced Wharton jelly mesenchymal stem cells toward chondrogenesis in an osteoarthritis model

محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 21، شماره: 7
سال انتشار: 1397
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 123

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لینک ثابت به این مقاله:
https://civilica.com/doc/1295275

شناسه ملی سند علمی:

JR_IJBMS-21-7_014

تاریخ نمایه سازی: 27 مهر 1400

چکیده مقاله:

Objective(s): This study aimed to determine the collagen type II (COL۲) and SOX۹ expression in interleukin growth factor (IGF-۱)-induced Wharton’s Jelly mesenchymal stem cells (WJMSCs) and the level of chondrogenic markers in co-culture IGF۱-WJMSCs and IL۱β-CHON۰۰۲ as osteoarthritis (OA) cells model. Materials and Methods: WJMSCs were induced with IGF۱ (۷۵, ۱۵۰, and ۳۰۰ ng/ml) to enhance their chondrogenesis capability. The gene expression of SOX۹ and COL۲ was evaluated with quantitative RT-PCR. Furthermore, IGF۱-WJMSCs were co-cultured with IL۱β-CHON۰۰۲ cells in varied ratios (۱:۲, ۱:۱, ۲:۱). Chondrogenic markers ADAMTS۱, ADAMTS۵, MMP۳, MMP۱, and RANKL were measured with ELISA. Results: The IGF۱-WJMSCs had an increased expression of COL۲ and SOX۹. ADAMTS۱, ADAMTS۵, MMP۱, MMP۳, and RANKL levels were decreased in the co-culture IGF۱-WJMSCs and IL۱β-CHON۰۰۲. Conclusion: The IGF۱-induced WJMSCs were capable to enhance chondrogenesis, indicated by increased expression of SOX۹ and COL۲ and decreased expression of ADAMTS۱, ADAMTS۵, MMP۳, MMP۱, and RANKL. These findings can be further used in the osteoarthritis treatment.

کلیدواژه ها:

Chondrogenesis ، IGF-۱ ، Mesenchymal stem cell MMPs ، Osteoarthritis

نویسندگان

Wahyu Widowati

Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung ۴۰۱۶۴, West Java, Indonesia

Ervi Afifah

Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung ۴۰۱۶۳, West Java, Indonesia

Tjandrawati Mozef

Research Center for Chemistry, Indonesian Institute of Sciences, Serpong, Indonesia

Ferry Sandra

Department of Biochemistry and Molecular Biology, Faculty of Dentistry, Trisakti University, Jakarta, Indonesia

Rizal Rizal

Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung ۴۰۱۶۳, West Java, Indonesia

Annisa Amalia

Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung ۴۰۱۶۳, West Java, Indonesia

Yukko Arinta

Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung ۴۰۱۶۳, West Java, Indonesia

Indra Bachtiar

Stem Cell and Cancer Institute, Jakarta ۱۳۲۱۰, Indonesia

Harry Murti

Stem Cell and Cancer Institute, Jakarta ۱۳۲۱۰, Indonesia

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