Evaluation of antioxidant and antimicrobial activities of Echium italicum L. extracts, (leaf, flower, stem and seed)

In antioxidant activities, by DPPH test, IC۵۰ values ۶۳.۱۱±۱.۴۵, ۱۰۲.۸۱±۱.۰۸, ۲۰۱.۲۴±۲.۱۸ and ۱۳۱.۶۲±۰.۴۶ μg/ml were obtained for leaf, flower, stem and seed extract respectively, comparable to that of BHT (IC۵۰ = ۱۹.۷۲± ۰.۸۰ μg/ml). In beta-carotene-linolenic acid test, inhibition percentages of %۵۷.۲۲, %۴۸.۷۴, %۴۸.۴۸ and %۴۴.۸۵ were recorded for leaf, flower, stem and seed extracts, respectively, comparable to that of BHT (%۸۹.۴۳). In total phenolic compounds contents of the extracts equivalent to galic acid were evaluated ۶۸.۸۲, ۳۰.۲۵, ۱۶.۷۶ and ۲۷.۸۶ μg/mg for leaf, flower, stem and seed extracts,respectively.In antimicrobial activity by disk diffusion method, leaf extract, inhibited paratyphoid microbe with a ۱۲ mm corona by at least ۶۰.۲۵μg/ml dilution, stem extract inhibited pneumonia microbe by ۱۸ mm corona at least ۵۰۰μg/ml dilution and flower extract inhibited two before microbes with a ۱۲ and ۱۳mm coronas by at least ۵۰۰ and ۲۵۰μg/ml dilutions respectively.